Wet Lab
1. RNA work
In vitro transcription - includes
preparative page & UV shadowing procedure
Analytical
Urea PAGE - procedure
HPLC
purification and work-up
Lyophilization
- site procedure
Sep
Pak - desalting of oligonucleotides
2. Protein Work
Electrophoresis Stocks for Protein
analysis
PAGE
stock solutions
T7
purification (non-HIS)
MT
purification (IG)
3. Molecular Biology
Cell
growth/transformation
Various vector
maps
4. Crystallization
Crystallization - stock solutions
Cubic
Lipid Phase crystallization
Crystallization
setup (form)
Crystal inventory form
Synchrotron packing list
Jolanta's DLS presentation
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Lab Protocals and Documentation (password required)