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Publications

2008

Chernyakov I, Whipple JM, Kotelawala L, Grayhack EJ, Phizicky EM (2008) Degradation of several hypomodified mature tRNA species in Saccharomyces cerevisiae is mediated by Met22 and the 5'-3' exonucleases Rat1 and Xrn1. Genes Dev, 22:1369-80 Link to PubMed

Kotelawala L, Grayhack EJ, Phizicky EM (2008) Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species. Rna, 14:158-69 Link to PubMed

2007

Wilkinson ML, Crary SM, Jackman JE, Grayhack EJ, Phizicky EM (2007) The 2'-O-methyltransferase responsible for modification of yeast tRNA at position 4.Rna, 13:404-13 Link to PubMed

Malkowski MG, Quartley E, Friedman AE, Babulski J, Kon Y, Wolfley J, Said M, Luft JR, Phizicky EM, DeTitta GT, Grayhack EJ (2007) Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing. Proc Natl Acad Sci U S A, 104:6678-83 Link to PubMed

Jackman JE, Kotelawala L, Grayhack EJ, Phizicky EM (2007) Identification and characterization of modification enzymes by biochemical analysis of the proteome. Methods Enzymol, 425:139-52 Link to PubMed

White, M.A., Clark, K.M., Grayhack, E.J. and Dumont, M.E. (2007) Characteristics affecting expression and solubilization of yeast membrane proteins. J Mol Biol, 365:621-36 Link to PubMed

2006

Phizicky, E.M., and Grayhack, E.J. (2006) Proteome-scale analysis of biochemical activity. Crit Rev Biochem Mol Biol, 41:315-27 Link to PubMed

Alexandrov A, Chernyakov I, Gu W, Hiley SL, Hughes TR, Grayhack EJ, Phizicky EM (2006) Rapid tRNA decay can result from lack of nonessential modifications. Mol Cell, 21:87-96 Link to PubMed

2005

Gu, W., Hurto, R.L., Hopper, A.K., Grayhack, E.J. and Phizicky, E.M. (2005) Depletion of Saccharomyces cerevisiae tRNA(His) guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m(5)C. Mol Cell Biol, 25:8191-201 Link to PubMed

Alexandrov, A., Grayhack, E.J. and Phizicky, E.M. (2005) tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p. Rna, 11:821-30 Link to PubMed

Ma, X., Yang, C., Alexandrov, A., Grayhack, E.J., Behm-Ansmant, I. and Yu YT (2005) Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism. Embo J, 24:2403-13 Link to PubMed

Gelperin, D.M., White, M.A., Wilkinson, M.L., Kon, Y., Ku,ng, L.A., Wise, K.J., Lopez-Hoyo, N., Jiang, L., Piccirillo, S., Yu, H., Gerstein, M., Dumont, M.E., Phizicky, E.M., Snyder, M. and Grayhack, E.J. (2005) Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes Dev, 19:2816-26 Link to PubMed

2004

Alexandrov, A., Vignali, M., LaCount, D.J., Quartley, E., de Vries, C., De Rosa, D., Babulski, J., Mitchell, S.F., Schoenfeld, L.W., Fields, S., Hol, W.G., Dumont, M.E., Phizicky, E.M. and Grayhack, E.J. (2004) A facile method for high-throughput co-expression of protein pairs. Mol Cell Proteomics, 3:934-8 Link to PubMed

2003

Van Slyke, C. and Grayhack, E.J. (2003) The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region. Nucleic Acids Res, 31:4597-607 Link to PubMed

2002

Phizicky, E.M., Martzen, M.R., McCraith, S.M., Spinelli, S.L., Xing, F., Shull, N.P., Van Slyke, C., Montagne, R.K., Torres, F.M., Fields, S. and Grayhack, E.J. (2002) Biochemical genomics approach to map activities to genes. Methods Enzymol, 350:546-59 Link to PubMed

2001

Grayhack, E. J. and Phizicky, E. M.(2001) Genomic Analysis of biochemical function. Curr. Opin. Chem. Biol. 5:34-39. Link to PubMed

1999

M. R. Martzen, S. M. McCraith, S. L. Spinelli, F. M. Torres, S. Fields, E. J. Grayhack and E. M. Phizicky(1999) A biochemical genomics approach for identifying genes by the activity of their products. Science 286: 1153-5 Link to PubMed

1997

Kuo, M.-H., E. Nadeau and E. Grayhack.(1997) Multiple phosphorylated forms of the Saccharomyces cerevisiae Mcm1 protein include an isoform induced in response to high salt concentrations. Molecular and Cellular Biology 17, 819-832. Link to PubMed

1994

Kuo, M.-H. and E. Grayhack(1994) A library of yeast genomic Mcm1 binding sites contains genes involved in cell cycle control, cell wall and membrane structure and metabolism. Molecular and Cellular Biology 14, 348-359. Link to PubMed

1992

Grayhack, E.(1992) The yeast alpha 1 and Mcm1 proteins bind a single strand of their duplex DNA recognition site. Molecular and Cellular Biology 12, 3573-3582. Link to PubMed