Barry M. Goldstein, Ph.D., M.D.

 

Associate Professor

Department of Biochemistry and Biophysics

Biophysics and Structural Biology Cluster

University of Rochester Medical Center, 601 Elmwood Ave, Rochester 14642

Phone (716) 275 5095, Fax (716) 275 6007

barry_goldstein@urmc.rochester.edu

Our laboratory uses the technique of macromolecular crystallography to study the binding of small molecules (ligands) to large molecules (proteins). Although we carry out a number of projects, the common goal is to identify ligand-protein interactions which determine how tightly the ligand binds to the protein 1) relative to other ligands (ligand affinity) and 2) relative to other proteins (ligand specificity). These studies have applications in the design of new drugs, the design of proteins that synthesize chemicals in an enviornmentally friendly way, and in the study of basic enzymatic reactions. Our work falls under the general headings of structural biology and structural chemistry. Links to projects are given below. Click here for links to publications and a description of the X-ray Diffraction facility.
Projects

Inosine monophosphate dehydrogenase: A major antitumor target

Cytochrome P450cam: Active site design

Dehydrogenase-catalyzed hydride transfer:

Mechanisms

Recent Publications:

Click here for a complete list of publications, patents and reprints.

Bahnson, B. J., Colby, T. D., Chin, J., Goldstein, B. M., Klinman, J. P. A link Between Protein Structure and Enzyme Catalyzed Hydrogen Tunneling. Proc. Nat. Acad. Sci. , 94: 12797-12802 (1997) . REPRINT *

Lesiak, K; Watanabe, K. A., Majumdar, A., Powell, J., Seidman, M., Vanderveen, K., Goldstein, B. M., Pankiewicz, K. W. Synthesis of Methylene Bis(phosphonate) Analogue of Mycophenolic Adenine Dinucleotide. A Glucuronidation Resistant MAD Analogue of NAD. J. Med. Chem. , 41:618-622 (1998) . REPRINT *

Colby, T. D.; Bahnson, B. J., , Chin, J., Klinman, J. P.; Goldstein, B. M. Active Site Modifications in a Double Mutant of Liver Alcohol Dehydrogenase: Structural Studies of Two Enzyme-ligand Complexes. Biochemistry , 37:9295-9304 (1998) . REPRINT*

Colby, T. D., Vanderveen, K., Strickler, M.D., Markham, G. D., Goldstein, B.M. Crystal Structure of Human Type II Inosine Monophosphate dehydrogenase: Implications for ligand binding and drug design. Proc. Nat. Acad. Sci. 96: 3531-3536 (1999) . REPRINT*

*REPRINTS are in the form of Adobe PDF files. Depending upon how your browser is set up, clicking on "REPRINT" will either open the manuscript directly in the browser, or download the PDF file to your machine for viewing. In either case, you will need Adobe Acrobat Reader, which can be downloaded and installed for free by going to : http://www.adobe.com/prodindex/acrobat/readstep.html

 

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