Table of Contents

• 1 Yeast as a Model Eukaryote

• 2 Information on Yeast

• 3 Yeast Strains

• 4 Growth and Life Cycles

• 5 The Yeast Genome

• 6 Genetic Nomenclature
  6.1 Chromosomal Genes
  6.2 Mitochondrial Gene
  6.3 Non-Mendelian Determinants

• 7 Genetic Analyses
  7.1 Overviews with Examples
  7.2 Tetrad Analysis
  7.3 Non-Mendelian Inheritance

• 8 Transformation
  8.1 Yeast Vector and DNA Fragments
  8.2 Synthetic Oligonucleotides
  8.3 Mitochondrial Transformation

• 9 Yeast Vectors
  9.1 YIp Vectors
  9.2 YEp Vectors
  9.3 YCp Vectors

• 10 Genes Important for Genetic Studies
  10.1 URA3 and LYS2
  10.2 ADE1 and ADE2
  10.3 GAL1 Promoter
  10.4 lacZ and Other Reporters

• 11 Manipulating the Genome In Vitro with Plasmids
  11.1 Cloning by Complementation
  11.2 Mutagenesis In Vitro
  11.3 Two-step Gene Replacement
  11.4 Gene Disruption and One-step Gene Replacement
  11.5 Plasmid Shuffle
  11.6 Recovering mutant alleles

• 12 Interactions of Genes
  12.1 Heterozygosity and Dominant-negative Mutations
  12.2 Intragenic Complementation
  12.3 Nonallelic Non-complementation
  12.4 Suppressors
  12.5 Synthetic Enhancement and Epistatic Relationships

• 13 Genomic Analysis

• 14 Analyses with Yeast Systems
  14.1 Two-hybrid Systems
  14.2 Yeast Artificial Chromosomes (YACs)
  14.3 Expression of Heterologous Protein in Yeast

• Key Words

• Bibliography