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School of Medicine and Dentistry

Facilities in Biophysics and Structural Biology

X-Ray Crystallography

The X-ray facility consists of a state-of-the-art Rigaku R-Axis IV image plate area detector mounted on a 2- q stage, RU200 rotating anode generator with mirror optics, and liquid a nitrogen-based crystal freezing device. Associated computational and graphics facilities include several SGI Indigo 2 workstations. Graphics and computational packages include SYBYL, QUANTA (with X-auto fit and X-power fit) and CNS.The X-ray facility is located in the Department of Biochemistry and Biophysics. The Director is Prof. Joseph Wedekind.

 

Varian Unity INOVA 600 NMR spectrometerNMR Spectroscopy

The Medical Center High Resolution NMR Facility houses a state-of-the-art Varian Unity INOVA 600 NMR spectrometer equipped with four RF channels, pulsed field gradient accessory and triple resonance probes. A GE Omega 400 (wide bore) spectrometer is also available in the facility. There are a few SGI andSUN Unix workstations available for data analysis and structure calculation. This facility is Located in the Department of Biochemistry and Biophysics, and is Directed by Prof. Scott Kennedy. In addition, the Chemistry department has a Varian Unity INOVA 500 spectrometer which is well suited for NMR studies of macromolecules, as well as an additional 500 MHz and two 400 MHz NMR spectrometers for student use. That facility is run by Prof. Thomas Krugh.

 

Electron Paramagnetic Spectrometer (EPR)EPR Spectroscopy

There are two research grade electron paramagnetic spectrometers (EPR) in the facility. A Bruker 200D-SRC with an X-band bridge has a wide bore magnet that accommodates a Janis cryostat. Sample temperature is continuously variable from 2K to 350K. Cavities are available for aqueous samples and electron nuclear double resonance (ENDOR) studies. A Varian E-12 spectrometer is equipped with both X- and Q-band bridges. Using a Janis cryostat, samples can be irradiated with x-rays, UV, or light and Q-band measurements taken at temperatures from 2K to 350K. The facility is run by Prof. William Bernhard.

 

Kornberg Medical Research BuildingURMC Core Facilities

URMC core facilities incorporate state-of-the-art instrumentation and are run by experts in their respective areas. Click on the above link for more detailed information. Included in our core are the following facilities:



> biosafety level-3

> pathology imaging

> cell sorting

> proteomics

> electron microscopes

> surface plasmon resonanace

> functional genomics

> transgenic

> microchemical protein/petpides

> vivarium




Protein Expression and Purification Equipment Cluster Resources

In addition to the crystallography, NMR, and EPR facilities run by our cluster, individual faculty members have a variety of instrumentation that they make available for use. Examples include:




> fermentation reactors
> fluorescence polarimeters
> Beckman FX liquid handling robots
> light scattering photometers (dynamic/static)
> MicroCal isothermal titration calorimeter (ITC)
> circular dichroism spectrometer
> cell sorters (FACS)
> confocal microscopes
> computational computer cluster
> phosphorimagers